The CLM-induced gamma-H2AX signal in WBC shows significant variation among individuals

<p><b>Copyright information:</b></p><p>Taken from "An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans"</p><p></p><p>Nucleic Acids Research 2007;35(5):e36-e36.</p><p>Published online 6 Feb 2007</p><p>PMCID:PMC1865071.</p><p>© 2007 The Author(s).</p> () Measurement of the gamma-H2AX signal in ten patients. Blood samples from ten patients were treated in duplicate with 0, 30 or 100 nM CLM. WBC were prepared, and the resulting gamma-H2AX signal was measured by flow cytometry. () No correlation between age and the gamma-H2AX signal. Blood samples from nine patients, with the age between 1 and 16 years, from a pediatric primary health care center, and blood samples from eleven patients 77–89 years old, admitted to the hospital, were treated in duplicate with 40 nM CLM. WBC were prepared, and the gamma-H2AX signal measured. A horizontal line indicates the mean gamma-H2AX signal in each age group. Error bars represent the difference in mean FITC signal intensity between double samples