The gamma-H2AX signal in blood lymphocytes irradiated with gamma radiation

<p><b>Copyright information:</b></p><p>Taken from "An optimized method for detecting gamma-H2AX in blood cells reveals a significant interindividual variation in the gamma-H2AX response among humans"</p><p></p><p>Nucleic Acids Research 2007;35(5):e36-e36.</p><p>Published online 6 Feb 2007</p><p>PMCID:PMC1865071.</p><p>© 2007 The Author(s).</p> () Measurement of IR-induced DNA damage by the gamma-H2AX assay and the comet assay in lymphocytes. Blood samples from a healthy subject were irradiated on ice with 0, 6, 20 or 60 Gy of gamma radiation. Lymphocytes were prepared from a sample of each irradiated blood sample and analyzed by the comet assay. The irradiated blood samples were incubated for 0.5 or 4.5 h at 37°C to allow H2AX phosphorylation. Lymphocytes were prepared and analyzed with the gamma-H2AX assay. () The IR-induced gamma-H2AX signal in lymphocytes shows significant variation among individuals. Blood from 20 patients were irradiated on ice with 8 Gy of gamma radiation and incubated for 0, 0.5 or 5 h at 37°C before preparation of lymphocytes. The lymphocytes were analyzed by the gamma-H2AX assay and by the comet assay. Error bars represent the difference in mean FITC signal intensity between double samples