Effect of Sox2 Oct-3/4 and Sox11 overexpression on Sox2 promoter activity

<p><b>Copyright information:</b></p><p>Taken from "Elevating the levels of Sox2 in embryonal carcinoma cells and embryonic stem cells inhibits the expression of Sox2:Oct-3/4 target genes"</p><p></p><p>Nucleic Acids Research 2007;35(6):1773-1786.</p><p>Published online 25 Feb 2007</p><p>PMCID:PMC1874607.</p><p>© 2007 The Author(s)</p> Schematic of the Sox2 promoter/reporter construct is shown. The gray boxes indicate critical enhancers, the basal Sox2 promoter is represented by a black box adjacent to the CAT reporter gene, and the relative HMG/POU cassette is indicated with an asterisk. F9 EC cells were transiently transfected and assayed as described in the Materials and methods section. The cells were transfected with 12 µg of the Sox2 promoter/reporter gene construct (Sox2 + US/DSE) plus 1 µg of the CMV-β-gal expression vector. In , 1 or 3 µg of either the Sox2 or Oct-3/4 expression vector was co-transfected. In , 3 µg of the indicated expression vector (Sox2, Oct-3/4 or Sox11) was co-transfected. Reporter activity was normalized to that of β-galactosidase and the activity of Sox2 + US/DSE (with no overexpression) was set to 1. The data shown represents the mean and standard deviation of duplicate samples from a representative experiment. Each experiment was repeated twice and similar results were obtained