Presence of p53 binding sites on p21 promoter is not required for down-regulation by mutant p53

<p><b>Copyright information:</b></p><p>Taken from "Cancer-derived p53 mutants suppress p53-target gene expression—potential mechanism for gain of function of mutant p53"</p><p></p><p>Nucleic Acids Research 2007;35(6):2093-2104.</p><p>Published online 7 Mar 2007</p><p>PMCID:PMC1874625.</p><p>© 2007 The Author(s)</p> () Schematic of promoter indicating the position of the p53 binding sites (S1 and S2) as described (). () Luciferase reporter assays were preformed by transfecting vector, wild-type p53, or the indicated p53 mutant cDNA constructs into H1299 cells as described, with the various promoter constructs. Transfections were carried out in triplicates and at least three independent times and the standard deviations are indicated