Identification of structural parameters that predict mRNA polysome loading in wild type and mutants

<p><b>Copyright information:</b></p><p>Taken from "On the functions of the h subunit of eukaryotic initiation factor 3 in late stages of translation initiation"</p><p>Genome Biology 2007;8(4):R60-R60.</p><p>Published online 17 Apr 2007</p><p>PMCID:PMC1896003.</p><p></p> The average translation state of mRNAs was plotted separately for wild-type and mutant plants after subdividing the transcriptome (5,101 genes with polysome loading data and full-length cDNA support) according to the following parameters: number of uAUGs in the 5' leader; the mutation did not cause a global reduction in mRNA transcript levels for uORF-containing mRNAs; length of the 5' leader in nucleotides (nt); similar to (c), but leaders were classified into subgroups according to the number of uORFs. For clarity, we plotted only the difference in translation state between and wild type (colored lines). The stippled line denotes the percentage of leaders in each length class (cumulative). Length of the 3' untranslated region. Length of the protein-coding region of the main ORF. Standard errors are shown. The asterisk indicates a significant difference between the translation state in wild type and mutant (< 0.05) according to a -test (unpaired, two-tail)