LamB1 translation after heat shock or intervention with ribosome scanning through 2A protease-dependent cleavage of eIF4G

<p><b>Copyright information:</b></p><p>Taken from "The leader region of Laminin B1 mRNA confers cap-independent translation"</p><p></p><p>Nucleic Acids Research 2007;35(8):2473-2482.</p><p>Published online 29 Mar 2007</p><p>PMCID:PMC1885646.</p><p>© 2007 The Author(s)</p> () LamB1 expression in MIM-R cells 4, 6 and 8 h after heat shock as detected by western blotting. () Firefly luciferase assay of MIM-R hepatocytes transfected either with pR-EMCV-F or pR-Lam-F bicistronic plasmids. Cells were exposed to heat shock 12 h post-transfection. 48 hours after transfection, Firefly luciferase activity was determined and normalized to the RNA level after reverse transcription and quantitation of cDNA. () Western blot analysis of MIM-R cells showing the cleavage of eIF4GI/II. MIM-R cells were transfected with wild-type 2A protease expressing plasmid (p2Awt) and lysed at the indicated times. () Firefly luciferase assay of MIM-R hepatocytes co-transfected with pR-F and either p2Amut or p2Awt. () Firefly luciferase assay of MIM-R cells co-transfected either with pR-EMCV-F or pR-Lam-F and p2Amut or p2Awt, respectively. Cells were lysed 48 h post-transfection and the Firefly luciferase activity was normalized to the RNA level after reverse transcription and quantitation of cDNA