Effect of the acceptor stem third base-pair variants on shRNA functional activity and strand-selectivity in both the S4tRNA and (+)tRNA backgrounds

<p><b>Copyright information:</b></p><p>Taken from "Optimization and characterization of tRNA-shRNA expression constructs"</p><p></p><p>Nucleic Acids Research 2007;35(8):2620-2628.</p><p>Published online 10 Apr 2007</p><p>PMCID:PMC1885648.</p><p>© 2007 The Author(s)</p> Dual luciferase assays of psiCHECK sense (open bars) and antisense (filled bars) targets. All constructs are normalized to the value of the corresponding empty promoter construct (S4tRNA or the U6) in combination with the relevant target (sense or anti-sense). All hairpins contain the BsrG1 loop except where an asterix (*) denotes the DC loop. (Lane 1) U6 shRNA (DC loop). (Lane 2) S4tRNA-3:70 C:U/DNt-A shRNA (BsrG1 loop). (Lane 3) S4tRNA-W shRNA (BsrG1 loop). (Lane 4) S4tRNA-3:70 C:C shRNA (DC loop). (Lane 5) S4tRNA-3:70 G:C flip/DNt-A shRNA (DC loop). (Lane 6) (+)tRNA-3:70 C:U/DNt-A shRNA (BsrG1 loop). (Lane 7) (+)tRNA-W shRNA (BsrG1 loop). (Lane 8) (+)tRNA-W shRNA (DC loop). (Lane 9) (+)tRNA-3:70 C:C shRNA (DC loop). (Lane 10) (+)tRNA-3:70 G:C flip/DNt-A shRNA (DC loop)