Important roles of multiple Sp1 binding sites and epigenetic modifications in the regulation of the methionine sulfoxide reductase B1 (MsrB1) promoter-4

<p><b>Copyright information:</b></p><p>Taken from "Important roles of multiple Sp1 binding sites and epigenetic modifications in the regulation of the methionine sulfoxide reductase B1 (MsrB1) promoter"</p><p>http://www.biomedcentral.com/1471-2199/8/39</p><p>BMC Molecular Biology 2007;8():39-39.</p><p>Published online 22 May 2007</p><p>PMCID:PMC1885803.</p><p></p>complexes. The control + lane was DNA that had been sonicated and pre-cleared with protein G beads. The control-lanes were processed according to the protocol, but did not have any Ab added to the samples. PCR were performed on isolated DNA using primers encompassing MsrB1 promoter region -169 to +76. A schematic map of the amplified DNA fragment (245 bp) containing Sp1 binding motifs and TSS position is illustrated as well