Overexpression of E2F1 can induce ARF and p53 and inhibit pol III transcription

<p><b>Copyright information:</b></p><p>Taken from "RNA polymerase III transcription is repressed in response to the tumour suppressor ARF"</p><p></p><p>Nucleic Acids Research 2007;35(9):3046-3052.</p><p>Published online 16 Apr 2007</p><p>PMCID:PMC1888803.</p><p>© 2007 The Author(s)</p> F9 cells were co-transfected in duplicate with pVA1 (1 μg), pGST (1 μg) and 15 μg of empty vector (control) or vector encoding full-length E2F1, as indicated. RNA and protein were isolated 48 h after transfection. () Western blot of exogenous E2F1 using anti-E2F1 (human) antibody KH95 and of endogenous p19 using anti-p19 antibody ab80, p53 using anti-p53 antibody 1C12 and actin, to confirm equal loading of protein, using anti-actin antibody C11. () Primer extension analysis of extracted RNA using primers specific for VA1 (upper panel), and GFP (lower panel)