Selective cytotoxicity of Pancratistatin-related natural alkaloids: evaluation of the activity of two new compounds-3

<p><b>Copyright information:</b></p><p>Taken from "Selective cytotoxicity of Pancratistatin-related natural alkaloids: evaluation of the activity of two new compounds"</p><p>http://www.cancerci.com/content/7/1/10</p><p>Cancer Cell International 2007;7():10-10.</p><p>Published online 5 Jun 2007</p><p>PMCID:PMC1892540.</p><p></p>s measured at excitation 400 nm and emission 505 nm. Activation of caspase-3 is a common event in cells undergoing apoptosis. The level of caspase-3 activity was calculated per microgram of protein and then displayed as a percent increase in fluorescence from caspase-3 activity in untreated cells (control). Values that are statistically significant to p < 0.05 are indicated with an asterisk. . Following treatment, Jurkat cells were incubated with a mitochondrial membrane potential (MMP) sensitive dye, and fluorescence was measured at excitation 574 nm and emission at 595 nm. Loss of MMP indicates permeable mitochondria which allows for numerous cytotoxic materials to leak into the cytosol, ending in apoptosis. Loss of MMP is represented as a drop in relative fluorescence units per 10,000 cells. Values that are statistically significant to p < 0.05 are indicated with an asterisk