Genetic analysis of the two zebrafish patched homologues identifies novel roles for the hedgehog signaling pathway-1

Use (A,B). At 72 hpf, mutants exhibit an eye phenotype where the pigmented epithelium is extended into the diencephalon. The similar phenotype described for the mutant is weaker compared to the mutant (C-E). The expression level of , a general readout for Hh activity, shows a mild increase in the mutant compared to wild type (F-H). The mutant shows a severely increased level of , where wild types, heterozygotes and mutants can be distinguished based on levels (I-K). Additional to the difference in the strength of the eye phenotype, the activation of the pathway is significantly higher in mutants compared to mutants. An increased expression level of confirms an activation of the Hh pathway in the mutant (L-N).<p><b>Copyright information:</b></p><p>Taken from "Genetic analysis of the two zebrafish patched homologues identifies novel roles for the hedgehog signaling pathway"</p><p>http://www.biomedcentral.com/1471-213X/8/15</p><p>BMC Developmental Biology 2008;8():15-15.</p><p>Published online 19 Feb 2008</p><p>PMCID:PMC2275722.</p><p>