Sensitive and robust gene expression changes in fish exposed to estrogen – a microarray approach-2

<p><b>Copyright information:</b></p><p>Taken from "Sensitive and robust gene expression changes in fish exposed to estrogen – a microarray approach"</p><p>http://www.biomedcentral.com/1471-2164/8/149</p><p>BMC Genomics 2007;8():149-149.</p><p>Published online 7 Jun 2007</p><p>PMCID:PMC1899179.</p><p></p>a nucleoside diphosphate kinase (nm23) after EEexposure measured with qPCR (green bars, male fish) or microarray (blue bars, male fish: red bars, female fish). Values are expressed as fold change (log) compared to control fish. Paired student's t-tests (single sided) were performed on the qPCR data to confirm/test the putative regulation suggested from microarray data. VTG, ZP3 and nm23 were confirmed to be significantly up-regulated in fish exposed to 10 ng/L (p = 0.001, 0.001 and 0.007 respectively, four biological replicates in each group). ZP3 and nm23, but not VTG were up-regulated in fish exposed to 0.87 ng/L (p = 0.0004, 0.006 and 0.5 respectively, eight biological replicates in each group) in accordance with the microarray data