Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase-0

<p><b>Copyright information:</b></p><p>Taken from "Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase"</p><p>http://www.virologyj.com/content/4/1/44</p><p>Virology Journal 2007;4():44-44.</p><p>Published online 24 May 2007</p><p>PMCID:PMC1904441.</p><p></p>pared and phosphorylated with isotopic [γP] label (*) as described in Methods. After melting and annealing, the RNA molecules self-anneal to form a six base-pair duplex with identical 5' overhangs. For Sym/Sub U, the correct base at N + 1 position, A, and one incorrect base, U, is shown (incorporated bases shown in lighter type). (B) List of the four Sym/Subs used in this study. Four similar synthetic RNAs were used. The first template base is shown in bold, and the correct base indicated in the right-hand column