Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase-1

<p><b>Copyright information:</b></p><p>Taken from "Biochemical characterization of the fidelity of poliovirus RNA-dependent RNA polymerase"</p><p>http://www.virologyj.com/content/4/1/44</p><p>Virology Journal 2007;4():44-44.</p><p>Published online 24 May 2007</p><p>PMCID:PMC1904441.</p><p></p>gle turnover, rapid quench reactions were performed and analyzed described in Methods. Product concentration of resulting 11 mer (in nM) as a function of time in seconds is shown for 75 (circles), 150 (squares) and 500 (triangles) μM UTP. Other reagents' concentrations were: 3D: 1 μM, Sym/Sub A: 1 μM (= 0.5 μM duplex). Data from each UTP concentration were fit to a single exponential. Amplitudes and observed reaction rates are given in Table 1. (B) Observed reaction rates as a function of UTP concentration. Data were fit to a hyperbola. and are given in Table 1