Repressor element-1 silencing transcription factor/neuronal restrictive silencer factor (REST/NRSF) can regulate HSV-1 immediate-early transcription via histone modification-2

<p><b>Copyright information:</b></p><p>Taken from "Repressor element-1 silencing transcription factor/neuronal restrictive silencer factor (REST/NRSF) can regulate HSV-1 immediate-early transcription via histone modification"</p><p>http://www.virologyj.com/content/4/1/56</p><p>Virology Journal 2007;4():56-56.</p><p>Published online 7 Jun 2007</p><p>PMCID:PMC1906746.</p><p></p>4, 9, and 10), and pCMVp73 (Lane 5, 6, 11, and 12). Lane 1–6: Ethidium bromide staining. Lane 7–12: plasmid-specific nucleosmal protected ladder detected by vector probe. Lane 13 and 14: Naked plasmid digestion control. . Analysis of nucleosomal formation on episomal plasmids. The nuclei from stable 293HEK-pICP22 were subjected to MNase digestion followed by Southern blot hybridization. Lane 1–3: ethidium bromide staining of the genomic DNA nucleosomal protected ladder. Lane 4–6: episomal plasmid-specific nucleosmal protected ladder detected by vector probe. . Examination of episomal status of plasmids in the stable cells harboring pICP4 by Southern hybridization. Lane 1. Total DNA purified from 1.28 × 10stable cells; Lane 2: 10 pg plasmid; Lane 3: 0.1 ng plasmid DNA; 4: 1 ng plasmid DNA; 5: 10 ng plasmid DNA