Cells were harvested on day 6 and restimulated with PMA plus ionomycin, followed by intracellular cytokine staining for IFN-γ and IL-17. Dot plots represent the IFN-γ and IL-17 expression among CD4 cells (percentages are shown). Data are representative of three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "TL1A–DR3 interaction regulates Th17 cell function and Th17-mediated autoimmune disease"</p><p></p><p>The Journal of Experimental Medicine 2008;205(5):1049-1062.</p><p>Published online 12 May 2008</p><p>PMCID:PMC2373838.</p><p></p
Proliferation was measured by [H]thymidine incorporation during the last 8 h of stimulation. (B) In ...
<p>Naïve T cells were cultured under Th17 polarizing conditions for 3 days +/− 10 ng/ml IL-1β. (A) I...
<p>Purified CD4<sup>+</sup> T (1×10<sup>6</sup>) cells from spleen (A–C) and LNs (A) were stimulated...
Single-cell suspensions were stimulated with PMA plus ionomycin with Golgi Plug for 4 h, stained, an...
Naive CD4 T cells were stimulated with plate-bound anti-CD3/CD28 antibody under the indicated condit...
<p><b>A,</b> Representative cytofluorometric analysis of IL-17A and IL-22 intra-cellular expression ...
<p>Naïve CD4<sup>+</sup> T cells were cultured under Th17-promoting conditions and analyzed. A) IL-1...
<p>(A) Purified CD4<sup>+</sup> T were stimulated with plate-bound anti-CD3 (5 µg/ml) and titrated d...
<p>CD4+CD25- T cells were stimulated in the presence of Th17 polarising cytokines for four days and ...
Proliferation of Th1 cells (A) and Th17 cells (B) was determined by [H]thymidine incorporation after...
Cells were evaluated for the expression of the indicated cytokines by intracellular cytokine stainin...
<p>Bulk Th1 cells (A) were activated as shown for 48 hours and the concentration of IFNγ in culture ...
Cells were then stimulated with PMA/ionomycin and intracellular staining for IL-21 versus IL-17A was...
(B) CD4 T cells from OT-II mice were differentiated into Th1, Th2, and Th17 lineages. On day 5 of cu...
<p>Purified CD4<sup>+</sup> T cells were stimulated with plate-bound anti-CD3 in the presence or the...
Proliferation was measured by [H]thymidine incorporation during the last 8 h of stimulation. (B) In ...
<p>Naïve T cells were cultured under Th17 polarizing conditions for 3 days +/− 10 ng/ml IL-1β. (A) I...
<p>Purified CD4<sup>+</sup> T (1×10<sup>6</sup>) cells from spleen (A–C) and LNs (A) were stimulated...
Single-cell suspensions were stimulated with PMA plus ionomycin with Golgi Plug for 4 h, stained, an...
Naive CD4 T cells were stimulated with plate-bound anti-CD3/CD28 antibody under the indicated condit...
<p><b>A,</b> Representative cytofluorometric analysis of IL-17A and IL-22 intra-cellular expression ...
<p>Naïve CD4<sup>+</sup> T cells were cultured under Th17-promoting conditions and analyzed. A) IL-1...
<p>(A) Purified CD4<sup>+</sup> T were stimulated with plate-bound anti-CD3 (5 µg/ml) and titrated d...
<p>CD4+CD25- T cells were stimulated in the presence of Th17 polarising cytokines for four days and ...
Proliferation of Th1 cells (A) and Th17 cells (B) was determined by [H]thymidine incorporation after...
Cells were evaluated for the expression of the indicated cytokines by intracellular cytokine stainin...
<p>Bulk Th1 cells (A) were activated as shown for 48 hours and the concentration of IFNγ in culture ...
Cells were then stimulated with PMA/ionomycin and intracellular staining for IL-21 versus IL-17A was...
(B) CD4 T cells from OT-II mice were differentiated into Th1, Th2, and Th17 lineages. On day 5 of cu...
<p>Purified CD4<sup>+</sup> T cells were stimulated with plate-bound anti-CD3 in the presence or the...
Proliferation was measured by [H]thymidine incorporation during the last 8 h of stimulation. (B) In ...
<p>Naïve T cells were cultured under Th17 polarizing conditions for 3 days +/− 10 ng/ml IL-1β. (A) I...
<p>Purified CD4<sup>+</sup> T (1×10<sup>6</sup>) cells from spleen (A–C) and LNs (A) were stimulated...