Autocorrelation analysis reveals widespread spatial biases in microarray experiments-7

<p><b>Copyright information:</b></p><p>Taken from "Autocorrelation analysis reveals widespread spatial biases in microarray experiments"</p><p>http://www.biomedcentral.com/1471-2164/8/164</p><p>BMC Genomics 2007;8():164-164.</p><p>Published online 12 Jun 2007</p><p>PMCID:PMC1913520.</p><p></p>oarray is from Spellman 1998 [27], and the Affymetrix YE6100 is from Cho 1998 [24]. Labeled cDNA prepared form the same RNA source of cells traversing the cell cycle was hybridized onto UHN and UMC microarrays. Despite the similar, or identical, underlying biology, the autocorrelation periods are different and reminiscent of each microarray design. Thus, the characteristic period of each microarray directly corresponds to the distances of the probes on the microarray as a function of the distance of the genes in the genome. The latter is shown for the UHN (B), UMC (C), and SMD (D) microarrays alongside the autocorrelation levels for the different gene distances. Probe placement data for the Affymetrix YE6100 microarray was unavailable. In the UMC microarray, the autocorrelation period is 1/4 of the probe placement period. This is due to the precise nature of the spatial bias associated with the specific microarray analyzed (see text for details). Complete correspondence between the two parameters could be observed in other experiments (not shown)