EcoTILLING for the identification of allelic variants of melon , a factor that controls virus susceptibility-3

<p><b>Copyright information:</b></p><p>Taken from "EcoTILLING for the identification of allelic variants of melon , a factor that controls virus susceptibility"</p><p>http://www.biomedcentral.com/1471-2229/7/34</p><p>BMC Plant Biology 2007;7():34-34.</p><p>Published online 21 Jun 2007</p><p>PMCID:PMC1914064.</p><p></p>ned into the binary vector pBIN61 between left (LB) and right (RB) borders of the Ti plasmid. The 35S promoter and terminator are indicated as 35S-P and 35S-T, respectively. () RT-PCR detection of MNSV accumulation in bombarded leaves. pBMα5 (Mα5) and pB264 (264) constructs were bombarded separately and in combination with pB4E-PI (4E-PI) or pB4E-Ved (4E-Ved) into leaves of . Two to three independent samples were included in the gel showed. Virus accumulation was assessed using RT-PCR two days post bombardment. C+ and C- indicate positive and negative controls of RT-PCR, respectively. C+ corresponds to leaves from susceptible melon bombarded with pBMα5 and pB264. In C-, RT-PCR was carried out with RNA from non-inoculated leaves