Expression of secreted pro-MMP2/9 and MMP2/9 in the cell free medium of HEY cell line grown as a monolayer or as spheroids over 7 days

<p><b>Copyright information:</b></p><p>Taken from "α2β1 integrin affects metastatic potential of ovarian carcinoma spheroids by supporting disaggregation and proteolysis"</p><p>http://www.carcinogenesis.com/content/6/1/11</p><p>Journal of Carcinogenesis 2007;6():11-11.</p><p>Published online 14 Jun 2007</p><p>PMCID:PMC1929068.</p><p></p> Serum-free medium from the cells were collected and concentrated as described in the Materials and Methods. 1 μg of protein from cells grown as a monolayer and 0.5 μg of protein from spheroids was resolved on a 10% polyacrylamide gel supplemented with 1% gelatin. The experiment was repeated on OVHS1 cells with similar results. Expression of secreted pro-MMP2/9 and MMP2/9 activity in the cell free medium of HEY cell line grown as a monolayer in the absence or presence of plasminogen (10 ng/ml, lanes 1 and 2 respectively) and as spheroids over 4 days in the absence or presence of 1:10 phenanthroline (2 mM, lanes 3 and 4 respectively)