Enhanced effect of microdystrophin gene transfection by HSV-VP22 mediated intercellular protein transport-0

<p><b>Copyright information:</b></p><p>Taken from "Enhanced effect of microdystrophin gene transfection by HSV-VP22 mediated intercellular protein transport"</p><p>http://www.biomedcentral.com/1471-2202/8/50</p><p>BMC Neuroscience 2007;8():50-50.</p><p>Published online 8 Jul 2007</p><p>PMCID:PMC1931604.</p><p></p>post-transfection, transfected cells were fixed and immunocytochemistry was performed with the monoclonal anti-dystrophin Amino Terminal. Magnification: × 100. At 48 h after transfection with pAVP22, pAMICDYS, pAVP22-MICDYS, cell supernatant were prepared from 3T3 cells and then added to C2C12 cells in a 35 mm dish. After 45 min, the cells were washed with PBS and processed for immunocytochemistry. (D) Transfected by supernatant with pAMICDYS; (E) Transfected by supernatant with pAVP22-MICDYS. Magnification: × 200