Tau phosphorylation by GSK-3β promotes tangle-like filament morphology-1

<p><b>Copyright information:</b></p><p>Taken from "Tau phosphorylation by GSK-3β promotes tangle-like filament morphology"</p><p>http://www.molecularneurodegeneration.com/content/2/1/12</p><p>Molecular Neurodegeneration 2007;2():12-12.</p><p>Published online 28 Jun 2007</p><p>PMCID:PMC1936422.</p><p></p> blots of GSK-3β phosphorylated tau. Panel A and B show two representative blots. Three repetitions of each phosphorylated tau concentration, ranging from 800 to 1.56 ng, were spotted on a blot, then probed with an anti-phosphorylation specific antibody (See Materials and Methods for details). To estimate non-specific background levels, each blot also included one spot of non-phosphorylated tau at each concentration. Following density analysis, the antibody titrations were plotted (Panels D and E) and 1/2 max concentrations were estimated. Anti-S422 antibody shown in Panel C, and its titration plot in Panel F, did not recognize phosphorylation and served as a negative control