PNRC is a unique nuclear receptor coactivator that stimulates RNA polymerase III-dependent transcription-1

<p><b>Copyright information:</b></p><p>Taken from "PNRC is a unique nuclear receptor coactivator that stimulates RNA polymerase III-dependent transcription"</p><p>http://www.jmolecularsignaling.com/content/2/1/5</p><p>Journal of Molecular Signaling 2007;2():5-5.</p><p>Published online 5 Jul 2007</p><p>PMCID:PMC1939705.</p><p></p>on plasmid, pSG5-RPC39, 24 h post-transfection, cells were harvasted and lysed, and 15 mg of total proteins were immunoprecipitated with antibodies against GFP (Clontech) or RPC39 (Santa Cruz Biotechnology). Specifically bound proteins to Protein A agarose beads were separated on a 10% SDS-PAGE and analysed by immunoblotting with either anti-RPC39 (, blot: anti-RPC39) or anti-GFP (, blot: anti-GFP) antibody, as previously described [2]. An aliquot of cell lysate equal to 100 μg of protein was included in each SDS-PAGE gel for Western blot to examine whether the crude protein extracts used for co-immunoprecipitation contains EGFP-PNRC and RPC39 proteins. The protein bands with molecular weights corresponding to those of RPC39 or EGFP-PNRC were indicated by arrows