ApoER2 expression increases Aβ production while decreasing Amyloid Precursor Protein (APP) endocytosis: Possible role in the partitioning of APP into lipid rafts and in the regulation of γ-secretase activity-1

<p><b>Copyright information:</b></p><p>Taken from "ApoER2 expression increases Aβ production while decreasing Amyloid Precursor Protein (APP) endocytosis: Possible role in the partitioning of APP into lipid rafts and in the regulation of γ-secretase activity"</p><p>http://www.molecularneurodegeneration.com/content/2/1/14</p><p>Molecular Neurodegeneration 2007;2():14-14.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1939850.</p><p></p>ted, anti-V5 antibody and probed for ApoER2 and APP with the anti-HA and anti-Myc antibodies, respectively. , input lysate. NB, not bound. (), cell extracts were prepared from untransfected ApoER2-expressing cells and processed as in . anti-Myc antibody does not immunoprecipitate ApoER2 by itself. , anti-Myc antibody do immunoprecipitate APP695Myc in single transfected cells. , anti-V5 antibody immunoprecipitation idoneity was confirmed by β-catenin-V5 precipitation from transfected N2a cells. (), independent lysates from ApoER2-expressing cells and from APP695-Myc-transfected cells were combined and the co-immunoprecipitation experiment was continued with the anti-Myc antibody as in . ApoER2-HA and APP695-Myc requires an context for interaction. Remnants of ApoER2 detection after stripping and reblot are indicated by an asterisk