ApoER2 expression increases Aβ production while decreasing Amyloid Precursor Protein (APP) endocytosis: Possible role in the partitioning of APP into lipid rafts and in the regulation of γ-secretase activity-5

<p><b>Copyright information:</b></p><p>Taken from "ApoER2 expression increases Aβ production while decreasing Amyloid Precursor Protein (APP) endocytosis: Possible role in the partitioning of APP into lipid rafts and in the regulation of γ-secretase activity"</p><p>http://www.molecularneurodegeneration.com/content/2/1/14</p><p>Molecular Neurodegeneration 2007;2():14-14.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1939850.</p><p></p>d triangle), 10 μg (open triangle) or 20 μg (open inverted triangle) of an ApoER2 coding plasmid were incubated with 1 nM I-labeled-anti-HA IgG or 5 nM I-labeled-diFeTf for 1 h at 4°C and then shifted to 37°C for the indicated times. The percent of ligand internalized at each time point is equal to the amount of ligand internalized divided by the total cell-associated ligand (see "Materials and Methods") Values are the mean average of triplicate determinations with S.E. indicated by . () Lysates were prepared from similarly transfected cells and equal amounts of protein were subjected to 10% SDS-PAGE. Blots were probed with anti-HA, anti-ApoER2, anti-actin or anti-transferrin receptor (TfR) antibodies. ApoER2 expression decreases APP695 but not transferrin receptor endocytosis rate