ApoER2 expression increases Aβ production while decreasing Amyloid Precursor Protein (APP) endocytosis: Possible role in the partitioning of APP into lipid rafts and in the regulation of γ-secretase activity-9

<p><b>Copyright information:</b></p><p>Taken from "ApoER2 expression increases Aβ production while decreasing Amyloid Precursor Protein (APP) endocytosis: Possible role in the partitioning of APP into lipid rafts and in the regulation of γ-secretase activity"</p><p>http://www.molecularneurodegeneration.com/content/2/1/14</p><p>Molecular Neurodegeneration 2007;2():14-14.</p><p>Published online 9 Jul 2007</p><p>PMCID:PMC1939850.</p><p></p>hich is based on the secretase-dependent cleavage of a γ-secretase-specific substrate conjugated with a fluorescent molecule. Data represent mean ± S.E.M of three experiments in duplicated, presented as fold values of the basal activity. * P < 0.05, ** P < 0.01. (), 50 μg of protein/lane were subjected to 12% SDS-PAGE and endogenous PS1 levels were determined by Western blot. In N2a cells almost all PS1 is detected as PS1-NTF but in CHO LRP1-null cells a significant amount of Holo-PS1 is also detected. ApoER2 expression increases PS1-NTF and decreases Holo-PS1 compared to control CHO LRP1-null cells. The expression of actin was determined in the same blot as a loading control. (), densitometric analysis of Western blots as in () confirms that total PS1 levels were slightly increased in CHO-ApoER2 cells compared to the pcDNA3 cells. However, a further increase in the PS1-NTF fraction is clearly observed in the Western blot