Expression of hereditary hemochromatosis C282Y HFE protein in HEK293 cells activates specific endoplasmic reticulum stress responses-4

<p><b>Copyright information:</b></p><p>Taken from "Expression of hereditary hemochromatosis C282Y HFE protein in HEK293 cells activates specific endoplasmic reticulum stress responses"</p><p>http://www.biomedcentral.com/1471-2121/8/30</p><p>BMC Cell Biology 2007;8():30-30.</p><p>Published online 24 Jul 2007</p><p>PMCID:PMC1947972.</p><p></p>apsigargin, and cytosolic extracts were prepared 24 h posttransfection. Ten-microgram of protein was assayed for cytochrome release (A), and caspase-3 activation (B) by western blotting (n = 3). C282Y HFE (M) induced cytochrome release and processing of procaspase-3 compared to EV, WT HFE or 2.5 μM thapsigargin treated cells. (C) To confirm equal loading blots were stripped and probed for β-Actin. (D) Measurement of caspase-3 activity in the lysates of HEK293 cells (1 × 10) transfected with control (empty vector), WT HFE or C282Y HFE expression vectors, and positive control of 2.5 μM thapsigargin was used. C282Y HFE significantly increased caspase-3 activity (*, p = 0.0363) (n = 3)