A flow cytometry technique to study intracellular signals NF-κB and STAT3 in peripheral blood mononuclear cells-0

<p><b>Copyright information:</b></p><p>Taken from "A flow cytometry technique to study intracellular signals NF-κB and STAT3 in peripheral blood mononuclear cells"</p><p>http://www.biomedcentral.com/1471-2199/8/64</p><p>BMC Molecular Biology 2007;8():64-64.</p><p>Published online 31 Jul 2007</p><p>PMCID:PMC1949834.</p><p></p>� (50 ng/mL) (A), sCD40L (50 ng/mL) (C) or IL10 (100 ng/mL) (E) for various periods (0 to 30 min). Data were representative of five experiments. pNF-κB and pSTAT3 were detected by ELISA (n = 5) in activated PBMCs nuclear extracts and the time dependant effect of the PBMCs stimulus was observed. PBMCs were stimulated for various periods of time (0 to 30 min) with or without IL1β (B), sCD40L (D) or IL10 (F). Statistical significance (wilcoxon paired test; p < 0.05) was represented by an asterisk (*). Data represented the mean (± SD) of five experiments