Distribution of immunogold particles indicating AR (A) or P2YR (B) in different intra- and extracellular compartments

<p><b>Copyright information:</b></p><p>Taken from "Co-localization and functional cross-talk between A and P2Y purine receptors in rat hippocampus"</p><p></p><p>The European Journal of Neuroscience 2007;26(4):890-902.</p><p>Published online Jan 2007</p><p>PMCID:PMC2121138.</p><p>The Authors (2007). Journal Compilation Federation of European Neuroscience Societies and Blackwell Publishing Ltd</p> Note that both receptors are enriched at synapses and astroglia, compared to all other compartments. Data, from single labelling experiments, report the localization of receptors as the density (gold particles/µm; mean ± SEM) in the different compartments in the vicinity of hippocampal glutamatergic synapses (i.e. asymmetric synapses on spines, see Materials and methods; randomly selected, from = 3 animals; five grids analysed for AR and six grids for P2YR); the areas of the compartments were measured by point analysis (see Materials and methods). The numbers of gold particles on each structure are, in order 323, 230, 1, 52, 24, 31, 15 for AR; 330, 311, 5, 112, 35, 31, 32 for P2YR. Areas analysed for each compartment are, in order 10.8, 7.7, 0.1, 1.1, 0.2, 0.6, 1.3, 1.0 µm for AR, 12.1, 8.8, 0.2, 2.1, 0.2, 0.9, 1.7, 0.8 µm for P2YR. (A) AR. ** < 0.01, one-way , all compartments compared to mitochondria (Dunnett's multiple comparison test). Synaptic cleft is significantly different compared to all other domains ( < 0.01, one-way , Tukey's multiple comparison test). Astrocytes and synaptic cleft columns showed higher receptor density ( < 0.05, Student's -test, two-tails, unpaired) compared to mitochondria. (B) P2YR; * < 0.05, ** < 0.01 all domains compared to mitochondria, one-way (Dunnett's multiple comparison test). Synaptic cleft is significantly different compared to all other domains ( < 0.001 vs. all except astrocytes, < 0.01 vs. astrocytes, One-way , Tukey's multiple comparison test)