Ancient intron insertion sites and palindromic genomic duplication evolutionally shapes an elementally functioning membrane protein family-13

<p><b>Copyright information:</b></p><p>Taken from "Ancient intron insertion sites and palindromic genomic duplication evolutionally shapes an elementally functioning membrane protein family"</p><p>http://www.biomedcentral.com/1471-2148/7/143</p><p>BMC Evolutionary Biology 2007;7():143-143.</p><p>Published online 20 Aug 2007</p><p>PMCID:PMC1999503.</p><p></p>t to the lower. A total of 54 AA sequences of eukaryotic G-protein beta and two beta-like AA sequences were analyzed [see Additional file ]. The outgroup was 7 bacterial WD-repeat protein AA sequences. Tunicate GNBs were underlined. AA sequences are inferred from the established genome data bases, JGI, NCBI genomes, and Ensembl. Alignment was carried out by the ClustalX 1.83 program. The gaps within nonpreserved regions were carefully deleted manually by the BioEdit program. The phylogenetic tree derived from the aligned data was then made by the Neighbor Joining Method in the Mega3 v3.1 program, using the Amino Poisson correction model with Gamma-distributed Rates among sites (Gamma parameter 2.0) and Bootstrap as the test of inferred branches (Repetition 500), including 471 sites with a pairwise deletion of Gaps/Missing Data. and Gbeta76C genes may be differently grouped from the vertebrate-type GNBs according to this phylogenetic tree. Those bacterial AA sequences of WD-repeat proteins were also referred from the NCBI microbial genome database. Abbreviations: GNB, G-protein beta. Rosfsp, .; Anavar, ; Nossp, .; Nospc, ; Glovio,