High-efficiency protein expression in plants from agroinfection-compatible expression vectors-5

<p><b>Copyright information:</b></p><p>Taken from "High-efficiency protein expression in plants from agroinfection-compatible expression vectors"</p><p>http://www.biomedcentral.com/1472-6750/7/52</p><p>BMC Biotechnology 2007;7():52-52.</p><p>Published online 27 Aug 2007</p><p>PMCID:PMC2014746.</p><p></p>All plasmids were based on the binary vector pCB301 backbone. T-DNA border sequences not shown in maps. Open boxes represent open reading frames; black block arrows; 35S promoter (35S Pr); black boxes, CaMV 3' terminator sequence; light grey boxes, 5' non-translated leader sequence (L); dark grey box, ribozyme (Rz); bent arrows, locations of TMV subgenomic promoters. The TMV vectors in pJL36 and pJL43 contain the full complement of TMV genes as well as an additional subgenomic promoter. TMV sequences 5' of the multiple cloning site (MCS) are from the U1 strain of TMV. Virus sequences 3' of the MCS are from the U5 strain of TMV. TMV transcripts are processed by a ribozyme to generate authentic TMV 3' ends. The sequence of the MCS in pJL 36 and 43 are presented. Restriction endonuclease recognition sequences are underlined. Because SapI is non-palindromic (GCTCTTC N1/4) both strands of the MCS of pJL 43 are presented for clarity