RT-PCR analysis of the relative expression levels of 9 genes expressed in shell forming cells and tissues of

<p><b>Copyright information:</b></p><p>Taken from "Dynamic expression of ancient and novel molluscan shell genes during ecological transitions"</p><p>http://www.biomedcentral.com/1471-2148/7/160</p><p>BMC Evolutionary Biology 2007;7():160-160.</p><p>Published online 10 Sep 2007</p><p>PMCID:PMC2034539.</p><p></p> Histone H1 acted as a control to ensure equal cDNA synthesis efficiency and template loading into the RT-PCR reaction. Coloured rectangles group periods in development that correspond to the occupation of distinct habitats. The yellow rectangle groups planktonic larval stages. The white rectangle indicates animals of a size that occupy CCA dominated habitats. The red rectangle groups juvenile and adult stages that occupy the undersides of coral bommies. Gene abbreviations are as follows: Has-histH1, histone H1; Has-ubfm1, ubiquitin fold modifier 1; Has-ferrt, ferritin; Has-calmbp1, calcium binding protein 1; Has-tsfgr1, trochophore shell field glycine rich 1; Has-cam1, calmodulin 1; Has-vm1, veliger mantle 1; Has-vm2, veliger mantle 2; Has-lustA, lustrin A; Has-som, sometsuke. * indicates that RNA was extracted from whole animals rather than biomineralising tissues specifically