Secretion of target proteins by strains containing additional copies of the endogenous calnexin gene ()

<p><b>Copyright information:</b></p><p>Taken from "Increase of calnexin gene dosage boosts the secretion of heterologous proteins by "</p><p></p><p>Fems Yeast Research 2007;7(7):1168-1180.</p><p>Published online 06 Jul 2007</p><p>PMCID:PMC2040192.</p><p>© 2007 Artes Technology GmbH Journal compilation © 2007 Federation of European Microbiological Societies</p> All strains were cultured in YPG. (a) Western blot of culture supernatants of AlgE1-HCNE and AlgE1-TEFP-HCNE supertransformants. (b) Western blot of EndoH-treated culture supernatants of IFNG-HCNE and IFNG-TEFP-HCNE supertransformants. The lane labeled Ec contains -derived interferon-γ. (c) Coomassie-stained SDS-PAGE gel loaded with 1 : 4 diluted culture supernatants of Conphys-HCNE and Conphys-TEFP-HCNE supertransformants before and after EndoH treatment. The arrow indicates full-length 51-kDa phytase, and the star a proteolytic degradation product of phytase. The lanes show target proteins secreted by parent strains (AlgE1.52-4, IFNG.23-2, and Conphys.3-68), supertransformants (numbered lanes), and one control strain supertransformed with the plasmid pPhleo (Conphys-Phleo). Images were quantified by densitometric scanning as described in ‘Materials and methods’. The relative amount of product present in each lane is indicated as a percentage of that in the parent strain