Synergizes with nontypeable to induce inflammation via upregulating TLR2-0

Ere treated with for 1, 3, 5 and 7 hours, and relative quantity of TLR2 mRNA expression was measured by Q-PCR analysis. (B).-induced TLR2 mRNA expression in lung epithelial cell A549. Cells were treated with , and relative quantity of TLR2 mRNA expression was measured by Q-PCR analysis 5 hours after treatment. (C) -induced TLR2 promoter activity. HeLa cells were transiently transfected with TLR2 luciferase reporter gene, treated with , and relative luciferase activity was measured 5 hours after treatment. (D) The effects of WT strain D39 and PLY-deficient strain PLN on -induced TLR2 expression. Cells were treated either with D39 or PLN, and mRNA expression of TLR2 was measured by Q-PCR analysis 3 hours after treatment. (E) - and PLY-induced TLR2 mRNA expression in middle ear cell HMEEC-1. Cells were treated with or PLY, and TLR2 mRNA expression was measured 5 hours after treatment by Q-PCR analysis. (F & G) - and PLY-induced TLR2 expressions in the lung and middle ear of the mice. Wild-type mice were intratracheally inoculated with or PLY for -induced pneumonia (F) and inoculated into the middle ear through tympanic membrane for -induced otitis media model (G). Relative quantity of TLR2 mRNA was measured from the lungs of inoculated mice 3 hours after inoculation and from the middle ears of inoculated mice 9 hours after inoculation. (H) The effect of PLY on -induced TLR2 expression was measured using WT strain D39 and PLY-deficient strain PLN in mice . WT mice were intratracheally inoculated with D39 or PLN, or purified PLY, and TLR2 mRNA expression was measured from the lungs of inoculated mice 9 hours after inoculation by Q-PCR analysis. (I) Protein expression of TLR2 is up-regulated following infection. TLR2 protein expression was measured from the lungs WT mice inoculated with by western blotting analysis 6 hours after inoculation (left panel), and band data was analyzed using Kodak Image analysis system and data was presented as Fold induction (right panel). Data are means ± S.D. (n = 3). *, < 0.05 compared with CON, **, < 0.01 compared with CON, ***, < 0.05 compared with D39, CON, control.<p><b>Copyright information:</b></p><p>Taken from "synergizes with nontypeable to induce inflammation via upregulating TLR2"</p><p>http://www.biomedcentral.com/1471-2172/9/40</p><p>BMC Immunology 2008;9():40-40.</p><p>Published online 29 Jul 2008</p><p>PMCID:PMC2515102.</p><p>