Fluorescent PLGA microspheres were captured by antigen presenting cells after intramuscular administration

<p><b>Copyright information:</b></p><p>Taken from "Tissue distribution of DNA-Hsp65/TDM-loaded PLGA microspheres and uptake by phagocytic cells"</p><p>http://www.gvt-journal.com/content/5/1/9</p><p>Genetic Vaccines and Therapy 2007;5():9-9.</p><p>Published online 20 Sep 2007</p><p>PMCID:PMC2042973.</p><p></p> To determine the leukocyte subsets involved in the uptake process, mice received a single intramuscular injection of 6-coumarin labeled microspheres containing DNA-Hsp65/TDM, vector/TDM or empty microspheres (without plasmid DNA and TDM). After 7 days, the draining lymph nodes cells were collected for FACS analysis (CD80, CD86, CD11b, CD11c and class II MHC). Control group was injected with saline. (A): Number of cells positive for cell surface molecules CD80, CD86 and MHC class II; *p < 0.05 versus control formulations (vector/DMT and empty). (B): MFI increase expressed as a percentage from pcDNA3-Hsp65/TDM and pcDNA3/TDM formulations, normalized to empty formulation. The values represented in the graphs are the mean ± SD of three experiments