HeLa cells expressing tet-inducible RNAi-resistant GFP-tagged Mps1 fusions were transfected with shRNA vectors and analyzed by immunofluorescence microscopy, immunoblotting, and time-lapse microscopy

(A) Immunofluorescence images showing kinetochore localization of wild-type Mps1 (WT) and the catalytically inactive D664A mutant (KD). (B) Immunoblot showing repression of the endogenous Mps1 and induction of the RNAi-resistant GFP-tagged proteins. (C) Bar graph quantifying the mitotic phenotypes observed after repression of endogenous Mps1; whereas Mps1 WT rescues the RNAi defect, Mps1 KD exacerbates the phenotype. At least 132 cells were scored in each category. Bar, 5 μm.<p><b>Copyright information:</b></p><p>Taken from "Mps1 kinase activity restrains anaphase during an unperturbed mitosis and targets Mad2 to kinetochores"</p><p></p><p>The Journal of Cell Biology 2008;181(6):893-901.</p><p>Published online 16 Jun 2008</p><p>PMCID:PMC2426934.</p><p>