Calcium-sensitive regulation of monoamine oxidase-A contributes to the production of peroxyradicals in hippocampal cultures: implications for Alzheimer disease-related pathology-3

<p><b>Copyright information:</b></p><p>Taken from "Calcium-sensitive regulation of monoamine oxidase-A contributes to the production of peroxyradicals in hippocampal cultures: implications for Alzheimer disease-related pathology"</p><p>http://www.biomedcentral.com/1471-2202/8/73</p><p>BMC Neuroscience 2007;8():73-73.</p><p>Published online 16 Sep 2007</p><p>PMCID:PMC2048967.</p><p></p>, [Ca], and on ROS production was examined. (, the relative fluorescence intensity used for this set of ROS experiments was increased intentionally so as to avoid a "floor" effect, . so that a in ROS production could be demonstrated in CB-28K-overexpressing cells). (B) MAO-A and MAO-B activities were assessed radioenzymatically in corresponding cell lysates (*< 0.05 versus control levels). (C) SDS-PAGE-resolved total cellular proteins were probed for CB-28K overexpression and for the expression of MAO-A and β-actin (used as a loading control). (D) Densitometry graph representing gene expression (relative to β-actin expression) determined by semi-quantitative RT-PCR, represented in (E). Data are presented as mean ± SD