Effect of SN on LPS-induced production of pro-inflammatory factors and their mRNA expression

<p><b>Copyright information:</b></p><p>Taken from "Sinomenine, a natural dextrorotatory morphinan analog, is anti-inflammatory and neuroprotective through inhibition of microglial NADPH oxidase"</p><p>http://www.jneuroinflammation.com/content/4/1/23</p><p>Journal of Neuroinflammation 2007;4():23-23.</p><p>Published online 19 Sep 2007</p><p>PMCID:PMC2064906.</p><p></p> Enriched microglia cells were pretreated with vehicle or SN (10, 10, and 10M) for 30 min before LPS (10 ng/ml) stimulation. Effects of SN are shown on LPS-induced production of superoxide (, expressed as % of control); and on intracellular ROS (, expressed as absolute absorbance). Extracellular superoxide was measured as SOD-inhibitable reduction of WST-1, and intracellular ROS was determined by probe DCFH-DA. Supernatant was collected at 24 h for nitrite assay (), at 3 h for TNF-α assay (), and at 24 h for PGEassay (). RNA were extracted at 3 h after LPS stimulation; the effect of SN, at sub-picomolar concentrations, on LPS-induced iNOS, TNF-α, and COX-2 mRNA expression (respectively, open bars) are shown. Results are expressed as mean ± SE for three independent experiments performed in triplicate. *< 0.05, **< 0.01 compared with the LPS-treated cultures