An AICD-based functional screen to identify APP metabolism regulators-3

<p><b>Copyright information:</b></p><p>Taken from "An AICD-based functional screen to identify APP metabolism regulators"</p><p>http://www.molecularneurodegeneration.com/content/2/1/15</p><p>Molecular Neurodegeneration 2007;2():15-15.</p><p>Published online 24 Aug 2007</p><p>PMCID:PMC2071909.</p><p></p>83-Gal4, and sAPPα levels compared to cells transfected with empty vector. (B) Quantification of Western blot densitometry in panel A. (C) ADAM17 transient over-expression significantly increases ADAM17, AICD-Gal4, C83-Gal4, and sAPPα levels. (D) Quantification of Western blot densitometry in panel C. (E) Transient over-expression of individual secretase genes increases AICD-Gal4 mediated luciferase activity. Luciferase was normalized to transfection efficiency, by dividing by luciferase activity. Individual secretase over-expression plasmids were co-transfected with pRL-SV40 plasmid, expressing luciferase. Bars represent the mean normalized luciferase activity of four independent trials and error bars represent standard errors. Statistical significance was determined using two-sample, one-tail t-tests to compare each secretase gene with the empty vector, followed by sequential Bonferroni procedure to adjust for multiple comparisons. * indicates p < 0.05; ** indicates p < 0.01