A rapid and sensitive bioassay to measure bone morphogenetic protein activity-7

<p><b>Copyright information:</b></p><p>Taken from "A rapid and sensitive bioassay to measure bone morphogenetic protein activity"</p><p>http://www.biomedcentral.com/1471-2121/8/41</p><p>BMC Cell Biology 2007;8():41-41.</p><p>Published online 19 Sep 2007</p><p>PMCID:PMC2094707.</p><p></p> for the reverse transcriptase-polymerase chain reaction (RT-PCR) to examine the expression of BMP and BMPRs. G3PDH gene expression was used as an internal control. PCR products were separated in 1% agarose gel and visualized after ethidium bromide staining. (A) Representative RT-PCR analysis of total RNA using specific primers for BMP-2, BMP-4, BMP-6 and BMP-7. (B) Representative RT-PCR analysis of total RNA using specific primers for BMP type I receptors (ALK2, ALK3, ALK6) and BMP type II receptors (BMPR-II, ActR-IIA, ActR-IIB). Similar results were obtained in two separate experiments