Inhibition of heme oxygenase attenuates guanosine-enhanced NGF-dependent neurite outgrowth in PC12 cells

<p><b>Copyright information:</b></p><p>Taken from "Guanosine stimulates neurite outgrowth in PC12 cells via activation of heme oxygenase and cyclic GMP"</p><p></p><p>Purinergic Signalling 2005;1(2):161-172.</p><p>Published online Jan 2005</p><p>PMCID:PMC2096532.</p><p></p> PC12 cells were cultured with NGF (40 ng/ml) or NGF (40 ng/ml) plus guanosine (300 µM) as described in Figure . Some cultures were treated with the selective inhibitor of heme oxygenase zinc protoporphyrin-IX (0.01Y1 µM) for 48 h and the number of cells bearing one or more neurites was determined as described in Figure 1. Open bars: NGF treatment, closed bars: NGF plus guanosine treatment. Cultures treated with guanosine plus NGF had a significantly ( < 0.01 two-way independent ANOVA) greater proportion of neurite-bearing cells than cultures treated with NGF alone. Zinc protoporphyrin-IX significantly decreased (** < 0.01) the neurite growth from cultures treated with guanosine plus NGF, but had no significant effect on neurite outgrowth in cultures treated with NGF alone. Data represent the mean ± SEM of 12 determinations from two replicate experiments