Calcium responses elicited by 2MeSADP stimulation in single cerebellar astrocytes

<p><b>Copyright information:</b></p><p>Taken from "Cerebellar astrocytes co-express several ADP receptors. Presence of functional P2Y-like receptors"</p><p></p><p>Purinergic Signalling 2005;1(2):153-159.</p><p>Published online Jan 2005</p><p>PMCID:PMC2096534.</p><p></p> Cells were loaded with fura-2 and the time-course of fluorescence changes recorded from cells after stimulation with 10 µM of the agonist for 40 s as described in Materials and methods. Where indicated, before cells were stimulated with 2MeSADP, they were preincubated for 3 min with 10 µM MRS-2179 in the absence (middle recordings) or presence of 100 µM 2MeSAMP (right recordings). Figure shows the calcium responses of three cells, which are representative of the cell subpopulations found with respect to their sensitivity to MRS-2179 antagonism (represented in the diagram, = 300 cells tested from three different cultures)