Verifications of microarray results by quantitative real-time PCR and example of oligonucleotide selection for highly homologous genes

<p><b>Copyright information:</b></p><p>Taken from "Selection of long oligonucleotides for gene expression microarrays using weighted rank-sum strategy"</p><p>http://www.biomedcentral.com/1471-2105/8/350</p><p>BMC Bioinformatics 2007;8():350-350.</p><p>Published online 19 Sep 2007</p><p>PMCID:PMC2099447.</p><p></p> The bar graph indicates mRNA abundance ratios between two developmental stages (schizont/trophozoite) of the IDC for 10 genes measured by microarray and by real-time RT-PCR. The expression data were obtained using the total RNA isolations from the trophozoite and schizont stage. Each measurement was carried three times and the standard error for each measurement is indicated. . The uniqueness score distributions along the two highly homologous histone 3 genes. The uniqueness is represented by the BLAST score of each 70 nt window along the histone genes (H3 and H3.3) to its second best target in the genome. The red arrow indicates the position of oligonucleotide selected by OligoRankPick in each gene