Statistical model for transgene copy number determination with internal reference gene design based on standard curve

<p><b>Copyright information:</b></p><p>Taken from "Statistical tools for transgene copy number estimation based on real-time PCR"</p><p>http://www.biomedcentral.com/1471-2105/8/S7/S6</p><p>BMC Bioinformatics 2007;8(Suppl 7):S6-S6.</p><p>Published online 1 Nov 2007</p><p>PMCID:PMC2099498.</p><p></p> A. Two-way ANOVA model with Ct number as dependent variable, and logarithm transformed concentration (logcon) and gene category (transgene or reference gene) as independent variables. The model can be used to derive ratio of copy number between transgene and reference gene and to test the equal amplification efficiency. B. The regression view of Ct plotted against logarithm 2 based transformation of relative concentration of genomic DNA template for both transgene and reference gene. Pink line is for transgene, and blue line is for control gene