Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line-8

<p><b>Copyright information:</b></p><p>Taken from "Paradoxical antiproliferative effect by a murine mammary tumor-derived epithelial cell line"</p><p>http://www.biomedcentral.com/1471-2407/7/184</p><p>BMC Cancer 2007;7():184-184.</p><p>Published online 1 Oct 2007</p><p>PMCID:PMC2129098.</p><p></p>C-conjugated primary antibody. Nuclei (blue staining) were identified using DAPI. Cytokeratin was undetectable in LM-234mf (B), whereas strongly expressed in LM-234ep (D), as revealed by immunohistochemistry using an anti-Pan cytokeratin antibody. Magnification bar, 100 μm. Cell lysates (25 μg/lane) were analyzed using Western blot for cytokeratins (E) and αSMA (F). The cytokeratins detected with the anti-pan antibody used range between 40 and 68 kDa. The band immunodetected for αSMA has an approximate molecular weight of 42 kDa. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) was used as a loading control