Design of a novel quantitative PCR (QPCR)-based protocol for genotyping mice carrying the neuroprotective Wallerian degeneration slow () gene-5

<p><b>Copyright information:</b></p><p>Taken from "Design of a novel quantitative PCR (QPCR)-based protocol for genotyping mice carrying the neuroprotective Wallerian degeneration slow () gene"</p><p>http://www.molecularneurodegeneration.com/content/2/1/21</p><p>Molecular Neurodegeneration 2007;2():21-21.</p><p>Published online 30 Oct 2007</p><p>PMCID:PMC2147001.</p><p></p>d Tg79 rat lines. The triplication results in the mouse having one full copy of (shown in orange) and (shown in brown) at either ends if the triplicated region. Within the triplicated region are 2 copies of an in frame fusion of the N70 amino acids of linked to the whole coding region of leading to the expression of 18 amino acids which are normally contained within the 5' untranslated region of (termed Wld-18 shown in red) which has been used for antibody targeting. Primers against the sequence were designed to amplify bases 810–885 in the Genbank sequence that correspond to a portion of the sequence in the chimeric product. Arrows indicate approximate site of amplification on the gene schematic. Figure modified from Gillingwater et al., 2006 [17]