Attenuation of WNT signaling by DKK-1 and -2 regulates BMP2-induced osteoblast differentiation and expression of OPG, RANKL and M-CSF-6

<p><b>Copyright information:</b></p><p>Taken from "Attenuation of WNT signaling by DKK-1 and -2 regulates BMP2-induced osteoblast differentiation and expression of OPG, RANKL and M-CSF"</p><p>http://www.molecular-cancer.com/content/6/1/71</p><p>Molecular Cancer 2007;6():71-71.</p><p>Published online 30 Oct 2007</p><p>PMCID:PMC2173906.</p><p></p>y Western blotting using Ab to FLAG, Dkk1-4 or GFP as indicated. β-actin levels (bottom) were determined as loading control. Untreated parental cells and cells transfected with GFP only (control) were included (bottom left). Protein size markers are indicated in kilodalton (kDa) to the left. , Secretion of Dkk1-4 into the cell culture supernatant. MOPC315.4 transfectants (1 × 10cells/cm) were cultured for 3 days and 10 μl CM were analyzed by Western blotting using Ab to Hisand Dkk1-4 as indicated. Note that Dkk2 was secreted as an apparent cleavage product