Effects of TNF-α gene deletion and Etanercept administration on TNFα levels

<p><b>Copyright information:</b></p><p>Taken from "Role of TNF-α in lung tight junction alteration in mouse model of acute lung inflammation"</p><p>http://respiratory-research.com/content/8/1/75</p><p>Respiratory Research 2007;8(1):75-75.</p><p>Published online 30 Oct 2007</p><p>PMCID:PMC2174464.</p><p></p> TNF-α production was evaluated in the pleural exudates () and lung tissues () collected at 4 h and 24 h after carrageenan administration using a colorimetric commercial ELISA kit. A significant production TNF-α was observed in pleural exudates () collected from WT mice. The absence of TNF-α receptor 1 gene in mice (TNF-αR1KO) as well as the treatment of WT mice with Etanercept significantly reduced the pleural exudate production of TNF-α. Similarly, a significant increase of the TNF-α () was observed in the lung tissues from carrageenan-injected WT mice at 4 and 24 hours after carrageenan. In the lung tissues from carrageenan-injected TNF-αR1KO mice as well as of WT mice which have received with Etanercept the TNF-α levels were significantly reduced in comparison to those of WT animals measured in the same conditions. Data are means ± SEM of 10 mice for each group. *< 0.01 . SHAM; °< 0.01 . carrageenan- WT group