Role of adipocyte-derived lipoprotein lipase in adipocyte hypertrophy-3

<p><b>Copyright information:</b></p><p>Taken from "Role of adipocyte-derived lipoprotein lipase in adipocyte hypertrophy"</p><p>http://www.nutritionandmetabolism.com/content/4/1/22</p><p>Nutrition & Metabolism 2007;4():22-22.</p><p>Published online 30 Oct 2007</p><p>PMCID:PMC2174487.</p><p></p>ubation with adipocyte induction reagents for 5 days. (A) Total RNA was isolated from cells and subjected to qRT-PCR analysis to determine relative LPL and leptin mRNA expression levels in siRNA treated and non-treated control cells. (B) Heparin-releasable LPL enzymatic activity was measured in siRNA treated and non-treated control cells as described in Fig. 1. (C) Intracellular lipid droplet formation was quantified by measuring adipocyte-associated Nile Red using fluorometry