Extensive ssDNA end formation at DNA double-strand breaks in non-homologous end-joining deficient cells during the S phase-4

<p><b>Copyright information:</b></p><p>Taken from "Extensive ssDNA end formation at DNA double-strand breaks in non-homologous end-joining deficient cells during the S phase"</p><p>http://www.biomedcentral.com/1471-2199/8/97</p><p>BMC Molecular Biology 2007;8():97-97.</p><p>Published online 26 Oct 2007</p><p>PMCID:PMC2174948.</p><p></p>ol at different repair times after irradiation. The number of DNA fragments was determined by PFGE and normalized to the number of DNA fragments at 0 h by the cold protocol. Error bars represent the SD of three experiments. *Denotes significant difference with paired t-test (p < 0.05) between warm and cold extraction. (B and C) BrdU corresponding to ssDNA was detected 4 h after irradiation in GM16147 cells treated with 50 μM NU7026 and cells were scored as belonging to one of the following categories: I. Weak BrdU staining in the whole nucleus; II. Increased BrdU staining in the nucleus compared to I or > 20 strong dots in the nucleus; III. Strong dots in the whole nucleus and background staining in the whole nucleus. At least 100 cells in three experiments were scored. Error bars represent the SD