Regulation of tight junction assembly and epithelial morphogenesis by the heat shock protein Apg-2-1

<p><b>Copyright information:</b></p><p>Taken from "Regulation of tight junction assembly and epithelial morphogenesis by the heat shock protein Apg-2"</p><p>http://www.biomedcentral.com/1471-2121/8/49</p><p>BMC Cell Biology 2007;8():49-49.</p><p>Published online 20 Nov 2007</p><p>PMCID:PMC2211299.</p><p></p>switching from low calcium to normal calcium medium, TER was measured at the indicated time points. Shown is a typical experiment using a control RNAi cell line (A), a ZO-1 RNAi cell line (B), and two Apg-2 RNAi cell lines (C and D). Values represent means +/- 1 SD of quadruplicates. (E) Paracellular permeability of fluorescent dextran was measured in the same cultures as those shown in panels A to D 3 days after the initiation of junction formation. Shown are the values obtained with 4 kD FITC dextran with tetracycline-treated cultures. Values represent means +/- 1 SD of quadruplicates. Diffusion of 70 kD dextran was also not affected by depletion of Apg-2 and ZO-1 (not shown)